SFB 1054 Seminar - Henrik Mei
German Rheumatism Research Center, Massenzytometrie, Berlin
26.04.2018 at 12:15
Title: Mass cytometry for immune cell profiling in chronic inflammation
Chronic inflammation and autoimmunity are mediated by aberrant differentiation and function of myeloid and lymphoid immune cells.
We employ high-dimensional mass cytometry to study cell types involved in the immune pathogenesis of chronic inflammation and autoimmunity, and to define disease- and patient-specific immune cell signatures that may help to facilitate precision medicine in chronic inflammatory diseases.
I will discuss results of a systematic comparison between blood cells of rheumatoid arthritis patients with matched controls and will outline several developments that help to generate high-quality mass cytometry data. These include different approaches to sample barcoding; dual fluorescent/metal-tagged antibodies which permit direct flow vs mass cytometry cross-platform comparisons and the pre-enrichment of rare cell subsets for downstream analyses by mass cytometry; the use of silver nanoparticles for sensitive detection of weakly expressed antigens, and the stabilization of cocktails of metal-labeled antibodies, suitable for longitudinal or multi-center immune phenotyping studies.
Venue:
TranslTUM, Center for Translational Cancer Research,
Johannes B. Ortner Forum, Room 22.0.1, EG
Ismaninger Str. 22, 81675 München
Host: Christina Zielinski (B10)
