SFB 1054 Seminar - Franziska Petermann
Lymphocyte Cell Biology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health (NIH), Bethesda, Maryland, USA.
22.03.2018 at 17:00
Title: The lncRNA NeST enhances Ifng expression by inducing chromatin loop formation
IFN-γ predominantly produced by T cells and NK cells, is a pleiotropic cytokine with both pro- and anti-inflammatory functions, uniquely regulating pathways involved in host defense and tumor surveillance. The expression of Ifng needs to be tightly regulated to ensure proper pathogen clearance while suppressing harmful, over-boarding immune responses. Previous studies have identified multiple regulatory elements at the mouse Ifng locus controlling its chromatin structure and transcriptional activity. However, the dynamics of how these regions including enhancers, silencers and insulators co-operate to control Ifng gene expression is still not completely elucidated. In this talk I will present our work on the non-coding antisense transcript, NeST, located 120 kb downstream of Ifng. We generated different mouse models to distinguish effects on Ifng expression caused by the NeST locus acting as an enhancer on the one hand and the NeST transcript on the other hand. Analysis of chromatin accessibility, CTCF binding and chromosomal conformation helped us to understand how NeST regulated the local chromatin architecture and Ifng expression. And finally, I will talk about the in-vivo relevance of NeST deficiency during a Toxoplasma gondii infection.
Venue:
BioMedical Center (BMC), Room N 01.017,
Großhaderner Str. 9, Planegg-Martinsried
Host: Thomas Korn (B06)
