SFB 1054 Seminar - Lukas Jeker
University of Basel, Switzerland
18.05.2017 at 12:15
Title: Genome engineering in primary T cells
Our group is interested in molecular mechanisms of gene regulation which are operational in T cell subsets known to be important for immune regulation. We previously demonstrated the importance of microRNAs for regulatory T cell (Treg) and T follicular helper cell (TFH) biology. However, despite major advances in miRNA research the analysis of target genes which are regulated by individual miRNAs remains challenging. To overcome these limitations we are developing CRISPR/Cas9-based gene editing tools. We developed a straightforward approach to ablate genes in up to 90% of primary T cells and to introduce precisely targeted single nucleotide polymorphisms (SNP) in up to 30% of the transfected primary T cells. We used gene editing-mediated allele switching to quantify homology directed repair (HDR), systematically optimize experimental parameters and map a native B cell epitope in primary T cells. We are currently applying these tools to study the molecular programs controlling T cell development.
Venue:
BioMedical Center (BMC), Room N 01.017,
Großhaderner Str. 9, Planegg-Martinsried
Host: Dirk Baumjohann (B12)
