SFB 1054 Seminar - Masahiro Ono

Title: Investigating time-dependent dynamic mechanisms of T cell regulation by single cell technologies

 We study temporally dynamic mechanisms underlying T cell differentiation and responses. This seminar will aim to show that, in order to understand immune regulation, it is indispensable to analyse time-dependent dynamics of the differentiation and function of individual T cells. Firstly, I will discuss fundamental problems in the current dogma of regulatory T cells, and show the importance of analysing temporally-dynamic mechanisms of T cell regulation [1, 2]. Next, I will show our findings by multidimensional analysis of single cell transcriptomes, and demonstrate a predicted dynamic mechanism for T cell regulation in vivo [3]. Furthermore, I will introduce a single-cell tool for time domain analysis, Timer of Cellular Kinetics and Activity(Tocky), and show temporally dynamic mechanisms of T cell regulation in vivo [4, 5]. Lastly, future perspectives will be discussed based on these investigations.

• Bending D, Ono M. (2018) From stability to dynamics: understanding molecular mechanisms of regulatory T cells through Foxp3 transcriptional dynamics. Clin Exp Immunol. doi: 10.1111/cei.13194.
• Ono M & Tanaka RJ. (2016) Controversies concerning thymus-derived regulatory T cells: fundamental issues and a new perspective. Immunol Cell Biol. Jan;94(1):3-10. doi: 10.1038/icb.2015.65.
• Bradley A, Hashimoto T, and Ono M. (2018) Elucidating T cell activation-dependent mechanisms for bifurcation of regulatory and effector T cell differentiation by multidimensional and single cell analysis, Front Immunol, doi 10.3389/fimmu.2018.01444.
• Bending D, Prieto-Martin P, Paduraru A, Ducker C, Marzaganov E, Laviron M, Kitano S, Miyachi H, Crompton, T, and Ono M. (2018) A timer for analyzing temporally dynamic changes in transcription during differentiation in vivo. J Cell Biol, doi 10.1083/jcb.201711048.
• Bending D, Paduraru A, Prieto-Martin P, Crompton T, and Ono M (2018) A temporally dynamic Foxp3 autoregulatory transcriptional circuit controls the effector Treg programme. EMBO J, 10.15252/embj.201899013.

Masahiro Ono - Website

Venue:

TranslaTUM
Small Auditorium (Johannes B. Ortner Forum)
Einsteinstr. 25, 81675 München

Host: Marc Schmidt-Supprian (A02)