SFB 1054 Seminar - Enrico Lugli
Title: Memory and effector T cell differentiation at single cell resolution
In response to immune activation, naive CD8+ T cells generate a large number of effector cells capable to migrating to peripheral tissues and kill infected cells. When antigen is cleared, a small population of antigen-specific T cells survives and differentiates into a pool of long-lived memory T cells that is highly diverse at the transcriptional, metabolic, epigenetic and functional levels. Single cell technologies are revolutionizing biology, and are opening new insights on how biological processes are regulated. We recently extended flow cytometry capability to measure up to 30-parameters on single cells are are combining it with single cell RNA sequencing to investigate T cell differentiation in physiology and pathology. In this talk, I will show how human effector differentiation is shaped by heterogeneity at the level of the preimmune repertoire and how a diverse progeny of memory T cells is present at the level of the immune microenvironment in human cancer and influences disease progression.
Host: Dietmar Zehn (B14)