SFB 1054 Seminar - Felix Meissner
Title: Decoding intercellular immune signaling networks by quantitative proteomics
The immune system is unique in its dynamic interplay between numerous cell types. However, a system-wide view of how immune cells communicate to protect against disease has not been established. Here, we developed and applied high-resolution mass spectrometry-based proteomics technologies to characterize primary human hematopoietic cell populations in steady and activated states at a depth of > 10,000 proteins. Protein copy numbers reveal a specialization of immune cells for ligand and receptor expression, thereby connecting distinct immune functions. By integrating total cellular and secreted proteomes, we establish a ‘social network’ of communicating cells and discover fundamental structures of intercellular information exchange as well as novel connections between cell types. Our publicly accessible (www.immprot.org) proteomic resource provides a framework for the orchestration of cellular interplay and a reference for altered communication associated with pathology.
Host: Thomas Brocker (B03)